Description
Intended Uses: This mouse Factor X total assay is for the quantitative determination of total Factor X and Xa in biological fluids. For research use only.
Principle of the Assay: Mouse Factor X will bind to the affinity purified capture antibody coated on the microtiter plate. Factor X, Xa, and Xa in complex with inhibitors will react with the antibody on the plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse Factor X primary antibody binds to the Factor X. Excess antibody is washed away and bound polyclonal antibody is then reacted with avidin conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm The amount of color development is directly proportional to the concentration of total Factor X in the sample.
Background: Factor X is a disulfide linked two-chain glycoprotein zymogen and is the precursor of the coagulation enzyme Factor Xa [1]. Factor X serves as the intersection of the intrinsic and extrinsic coagulation cascades and can be activated by either the extrinsic Factor VIIa / Tissue Factor compex or the intrinsic Factor IXa / Factor VIIIa complex. Factor Xa converts prothrombin to thrombin and is quickly inhibited by Antithrombin III in the presence of heparin